ZFIN Image: Acin-Perez et al., 2020, K

Image

Figure Caption

K Mitochondria isolated from previously frozen liver maintain intact electron transport system

A

Representative traces of oxygen consumption rate (OCR) of mouse liver mitochondria isolated from fresh or frozen tissue sustained by pyruvate + malate. Pyruvate + malate + ADP (PM + ADP), oligomycin (oligo), FCCP, and antimycin A + rotenone (AA + ROT) were sequentially injected to assess mitochondrial respiratory states.

B

Pyruvate + malate‐dependent state 3 (substrate plus ADP)/state 4 (substrate without ADP) in fresh and frozen liver mitochondria.

C

Quantification of maximal respiration rate (MRR) supported by pyruvate + malate in fresh and frozen liver mitochondria.

D

Representative traces of OCR of liver mitochondria isolated from fresh or frozen tissue supported by the Complex II substrate succinate + rotenone + ADP (SR + ADP).

E

Succinate + rotenone‐dependent state 3/state 4 in fresh and frozen liver mitochondria.

F

Quantification of the different bioenergetic parameters sustained by succinate + rotenone in fresh and frozen liver mitochondria.

G

Representative traces of OCR of liver mitochondria isolated from fresh or frozen tissue sustained by the Complex 1 substrate NADH + ADP.

H

NADH‐dependent state 3/state 4 in fresh and frozen liver mitochondria.

I

MRR driven by NADH in fresh and frozen liver mitochondria.

J

Representative traces of OCR of liver mitochondria isolated from fresh or frozen tissue starting in state 1 and sustained by substrates without ADP (state 4) and by substrates with ADP (state 3). Mitochondria were tested for CAT sensitivity.

K

Representative traces of OCR of liver mitochondria isolated from fresh or frozen tissue starting in state 1 and sustained by substrates with ADP (state 3). Mitochondria were tested for CAT sensitivity.

Data information: Panels (A, D, G, J, and K) are representative seahorse traces including four technical replicates. Biological replicates: (B and C), n = 4; (E and F), n = 6; and (H and I), n = 4. Every biological replicate represents the average of four technical replicates. Data are the mean ± SEM.

Acknowledgments

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