Figure 5

Figure 5

MV4-11 cells in zebrafish xenografts and drug treatment. Proliferation of MV4-11 cells in the zebrafish xenograft model with Ara-C and Aza treatments started from 2 dpf. The zebrafish xenograft embryos were dissociated (control group at 2 dpf and 5 dpf and treatment group at 5 dpf), and labeled leukemia cells were analyzed by ImageXpress Pico. (A) Total MV4-11 cells in zebrafish xenografts after Ara-C (500 µM), Aza (300 µM), and U0126 (10 µM) treatment. (B) Representative images of zebrafish xenografts with respective concentrations of drug treatment at 5 dpf. Quantitative values were means ± SEM from 5 to 8 independent groups of 3 xenografts in each group. Significance p values were determined as *p ≤ 0.05, **p ≤ 0.01 and ***p ≤ 0.001.