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Figure 4

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ZDB-IMAGE-210310-106
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Figures for Zizioli et al., 2021
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Figure 4

BCR-ABL1 induces a granulopoiesis expansion and granulocytes differentiation. Whole mount in situ hybridization (WISH) of scl at 24 hpf in Wild Type (WT) (A) and in Tg BCR-ABL1 (B). WISH at 28 hpf for pu1 (C,D); mpx1 (E,F); and L-plastin (G,H) respectively in WT and tg BCR-ABL1 fishes. The experiments were performed in duplicate; numbers in each panel represent total embryos used for the experiment and embryos with the result shown in the image. In each panel, red ovals indicate the positive signals of mpx1 and L-plastin in posterior blood island. Red arrows respectively indicate the mpx1 (F) and L-plastin (H) positive cells in the anterior part of the embryos. Statistical analysis of mpx1+ (I) and L-plastin+ (L) signals counted in posterior blood islands. Unpaired, two-tailed t-test **** p < 0.0001.

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