Figure 10.

Figure 10.

A strong correlation between red and green mEos 24 hpc suggests protein mixing within existing organelles. A, Schematic of the photoconversion and tracking assay. At 4 dpf, the whole larva was photoconverted, and then 6 and 24 hpc, the boxed regions were imaged. B–G, Imaging at 6 and 24 hpc of the whole larva shows gradual gain of new (green) mEos in the cell bodies of the pLLg (B, C), proximal axon (D, E), and distal axons (F, G) of the pLL. For D–G, boxed region is shown at a higher magnification on the right. Arrowheads point to mitochondria with strong green fluorescence and consistent red (converted) fluorescence as well. Arrows point to mitochondria that lack converted mEos. H, Correlational analysis of green and red mEos at 6 and 24 hpc (ANOVA with Tukey's HSD post hoc contrasts; n = 8). Green signal was converted into a mask to assay the presence of red (converted) fluorescence that overlapped. Proximal axon: 6 hpc: 0.70 ± 0.01; 24 hpc: 0.56 ± 0.02; Distal axon: 6 hpc: 0.77 ± 0.02; 24 hpc: 0.69 ± 0.03. Scale bar: 10 µm. Sample size on graph. All data are ±SEM.