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Fig. 3 Fig. 3. The cortisol-mediated [(Ca2+)i] increase is independent of glucocorticoid receptor. (a) Representative images of the developing trunk muscle (DTM) loaded with the ratiometric dye FURA-2AM and treated with either vehicle (CONTROL), cortisol (CORT), RU486 or a combination of cortisol and RU486 (RU486+CORT), as well as cortisol treatment of the DTM from GRKO zebrafish (GRKO + CORT). (b) Graph showing the ratiometric FURA-2AM intensity in the DTM treated with either cortisol, RU486 or a combination of cortisol and RU486 at 30 s post-cortisol exposure. (c) Graph showing the ratiometric FURA-2AM intensity in wildtype or GR knockout (GRKO) DTM at 30 s post-cortisol addition. All bars represent mean ± SEM (n = 4–5 independent embryos); bars with different letters are significantly different; one-way ANOVA; Holm Sidak post hoc test; p < 0.05.
Reprinted from Molecular and Cellular Endocrinology, 520, Das, C., Faught, E., Vijayan, M.M., Cortisol rapidly stimulates calcium waves in the developing trunk muscle of zebrafish, 111067, Copyright (2020) with permission from Elsevier. Full text @ Mol. Cell. Endocrinol.