ZFIN Image: Werner et al., 2021, Fig. 4

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Figure Caption

Fig. 4 Measurements of cell shape changes in the deep neuroectodermal layer and neural folds.

a-c Measurements of cell lengths (μM, Y axis) of mGFP-labeled cells at different positions relative to the ANP midline (μM, X axis). Measurements begin in the region fated to become the optic vesicles. d–f Measurements of the apico:basal surface ratio of mGFP-labeled cells at different positions relative to the ANP midline (μM, X axis). Measurements begin in the region fated to form the optic vesicles. The original scatter plot was fitted to a 3rd degree polynomial. Confidence intervals for the polynomial are 95% and were calculated with bootstrap sampling (n = 1000). 2 somites: n = 66 cells from three embryos, 5 somites: 77 cells from 3 embryos, 7 somites: 72 cells from 3 embryos. Color code: light blue = neuroectodermal cells of the deep layer of 2 som stage embryos that are not yet identifiable based on cellular morphology; red = cells that form the optic vesicles; purple = cells that form the neuroectodermal component of the neural folds.

Acknowledgments

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