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Figure 2.

ID: ZDB-IMAGE-210208-70
Publication: Alyenbaawi et al., 2021 - Seizures are a druggable mechanistic link between TBI and subsequent tauopathy
All Figures: Figures for Alyenbaawi et al., 2021

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Figure 2. Protein-only induction of Tau puncta in vivo detected in biosensor zebrafish.

Injections of synthetic Tau fibrils into Tau4R-GFP zebrafish induced GFP+ puncta in brains and spinal cord. (A,B) Inhibiting the proteosome with MG-132 enhanced the percentage of larvae bearing GFP+ inclusions in the brain following injection of tau-laden brain homogenate. (C) Synthetic human Tau proteins were fibrillized as confirmed via EM analysis. Human Tau fibrils were microinjected into the larval hindbrain at 2 days post-fertilization, and Tau inclusions were analyzed at 3 days post injections. (D) Tau aggregates were only observed after injection of Tau fibrils, not monomers (*p=0.0104, Kruskal Wallis test). (E) Tau aggregates (same data as D, presented as distribution of larvae that displayed various amounts of GFP+ puncta) appear only after injection of Tau fibrils, not monomers.

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