ZFIN Image: Hermanowicz et al., 2021, Figure 5.

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Figure 5.

The down-regulation of Bruton’s kinase (BTK) expression by MM-129. Phosphorylated BTK (pBTK) expression was determined by Western blot (a) and confocal microscopy (d) in DLD-1 and HT-29 cells treated with MM-129, roscovitine and 5-flurouracil in two concentrations: 1 µM and 3 µM for 24 h. The band staining was quantified by densitometry (b, c). Samples used for electrophoresis consisted of 20 µg of protein from six pooled cell extracts (n = 6). Data were presented as mean ± standard deviation (SD), and analysed using one-way analysis of variance (ANOVA). ***p<.001 vs. CON intensity of cytoplasmic/nuclear fluorescence was analysed in cell populations. Images of cells labelled with FITC were obtained using a 515LP emission laser and a 488/10 excitation laser (d).

Acknowledgments

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