|
Fig. 4 HK Mm are sufficient for increased neutrophil recruitment to the infection site. (A) Schematic of experiment for B–D. (B) Number of neutrophils at site of infection and tailfin wound at 4 hpi/w in mpx:GFP embryos. The groups are Wound (tailfin wound alone), Mock infection (PVP)/wound (injection of PVP into the somite alongside a tailfin wound), Mm infection/wound (injection of Mm into the somite alongside a tailfin wound) and HK Mm infection/wound (injection of HK Mm into the somite alongside a tailfin wound). Individual embryos are represented as lines joining their respective infection and tailfin wound neutrophil numbers. Data shown are mean ± SEM, n = 21 representative of three independent experiments. (C) Neutrophil numbers at the site of infection at 4 hpi. Data shown are mean ± SEM, n = 42 representative of three independent experiments. Statistics were determined using an unpaired t‐test. P values shown are as follows: ns = not significant and ***P < 0.001. (D) Neutrophil numbers at the site of tailfin wound at 4 hpi. Data shown are mean ± SEM, n = 42 representative of three independent experiments. Statistics were determined using an unpaired t‐test. P values shown are as follows: ns = not significant and ***P < 0.001.