ZFIN Image: Han et al., 2020, Figure 4

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Figure Caption

Figure 4

Generation of conditional sox10 KI alleles using minicircle donor and summary of our bidirectional KI strategy. (A) Schematic diagram of the KI strategy based on the minicircle Bi-FoRe donor derived from in vitro backbone elimination. (B) Representative z-stack confocal images of two 48 hpf founder embryos after injection of the sox10 minicircle Bi-FoRe donor vector together with the CRISPR/Cas9 system, showing red (representing forward insertion) and green (representing reverse insertion) fluorescent signals, respectively. Scale bar, 200 μm. (C) A graphical summary of the principle and applications of our Bi-FoRe KI strategy

Acknowledgments

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