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Figure 5

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ZDB-IMAGE-210128-142
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Figures for Zhou et al., 2021
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Figure Caption

Figure 5 Grass carp IFN1 from mucus inhibited the plaque formation of cultured cells infected with GCRV873. (A,B) Detection of the antiviral ability of grass carp IFN1 from the mucus of transgenic loach. 1, 5: added M199 medium containing 2% FBS; 2: added M199 medium containing 2% FBS and 20% mucus (v/v) from wide type (WT) loaches and infected with GCRV873 (viral titer: 1.0 × 102 PFU/mL); 3: added M199 medium containing 2% FBS and 20% mucus (v/v) from grass carp IFN1-expressing transgenic loaches and infected with GCRV873 (viral titer: 1.0 × 102 PFU/mL); 6: added M199 medium containing 2% FBS and 20% mucus (v/v) from WT loaches and infected with GCRV873 (viral titer: 1.0 × 102 PFU/mL); 7: added M199 medium containing 2% FBS and 20% mucus (v/v) from grass carp IFN1-expressing transgenic loaches and infected with GCRV873 (viral titer: 1.0 × 103 PFU/mL); 4, 8: added M199 medium containing 2% FBS and 20% mucus (v/v) from WT loaches. (C) Graph of plaque numbers of the Figure 5A. The experiments were performed in triplicate. Data were expressed as mean ± SD. * p < 0.05; ** p < 0.01.

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