SA exerts anti-proliferation and apoptosis-promoting effects in breast cancer cells. (A) Proliferation of the indicated cells treated with either certain concentrations (0–400 μg/ml) of SA for 48 h or certain time intervals (0, 24, and 48 h) of SA by CCK8 assays. (B) The influence of SA (0–200 μg/ml) on colony formation of MDA-MB-231, BT-549, and MCF-7 cells. (C) The apoptotic populations after a 48-h treatment of SA (0–200 μg/ml) in MDA-MB-231 and BT-549 cells. (D) The fluorescence photographs of SA-treated cells stained with Hoechst 33,258 dye (100 μg/ml). Scale bar: 50 μM. All values represent the mean ± SD (n = 3, *p < 0.05, **p < 0.01).
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