Fig. 2

Fig. 2

Light-Sheet Microscope Hardware Control: (A) Interaction of different components of the μSPIM Toolset-based setup in a typical acquisition setup. μSPIM Toolset provides control and synchronization of hardware through NI DAC with MicroManager controlling the camera and mechanical shutters through PCI and COM ports. (B) Traces of the command signals for a volume acquisition with 43 planes showing camera trigger (i.), laser shutter (ii.), X mirror signal (iii.), Z mirror (red) and Piezo (pink) signals for Scan Down (iv.), Scan Up (v.) and Bidirectional (vi.) acquisition modes generate by the μSPIM control software. (C) Magnification of boxed region in B with yellow region showing a single plane signal. Laser shutter signal is a result of a recording with Edge Masks enabled.