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Figure 3

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ZDB-IMAGE-201230-20
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Figures for Ketharnathan et al., 2020
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Figure 3

Stag mutations alter the number of gata1- and spi1-positive cells in 24 hpf zebrafish embryos. (A) Lateral views of gata1 expression in whole-mount embryos at 24 hpf; anterior to the left. gata1 expression is reduced in stag1anz204+/- and stag1anz204-/- embryos and is rescued upon injection of functional stag1a mRNA. gata1 expression is reduced in stag2bnz207+/- and stag2bnz207-/- embryos and is rescued upon injection of functional stag2b mRNA. Reduced expression is indicated by arrows. (B)spi1 expression in whole-mount embryos at 24 hpf. Left panels show lateral views and right panels show ventral views; anterior to the left. The number of spi1-positive cells is increased in stag1anz204+/- and stag1anz204-/- embryos (yellow arrows). spi1 expression in stag2bnz207 heterozygous embryos and stag2bnz207 homozygous mutant embryos is comparable to wildtype. Scale bars are 100 μm. The number of embryos is indicated in lower-right-hand corners. (C) mRNA levels of haematopoietic stem cell marker cmyb and erythroid or myeloid lineage markers in stag1anz204 and stag2bnz207 homozygous mutant embryos at 48 hpf. The bar graph shows the mean +/- one standard deviation. *P ≤ 0.05, **P ≤ 0.01; one-way ANOVA. Expression was normalized to the reference gene, b-actin.

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