ZFIN Image: Lee et al., 2020, Figure 2

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Figure Caption

Figure 2—figure supplement 4. Responses of individual IRa neurons to optogenetic stimulation of NPVF neurons in individual fish.

6-dpf Tg(npvf:eGFP); Tg(tph2:GCaMP6s-P2A-tdTomato) (A) and Tg(tph2:ReaChR-mCitrine); Tg(npvf:GCaMP6s-P2A-tdTomato) (B) animals were analyzed for normalized GCaMP6s fluorescence levels in IRa neurons pre- and post-optogenetic stimulation of NPVF neurons. Data from Figure 2 are displayed here as separate fish. Maximum intensity projection images (left) show GCaMP6s fluorescence averaged over the first 10 imaging frames post-stimulation and provide spatial information along the anterior/posterior and medial/lateral axes of the brain. Size of red or blue circle indicates magnitude of normalized GCaMP6s fluorescence increase or decrease, respectively, in response to optogenetic stimulation. Dot plots (right) show average normalized GCaMP6s fluorescence of all IRa neurons for each frame before and after optogenetic stimulation (red line). A, anterior; P, posterior; M, medial; L, lateral. n = number of IRa neurons imaged.

Acknowledgments

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