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Figure 7—figure supplement 3 Spatial quantification of fluorescence in situ hybridization signal along the dorsal-ventral axis for BMP target genes at shield stage in embryos treated as indicated, compared to untreated spatial distributions (black). Normalized intensities are shown, error bars represent standard error. (A) Embryos were injected with 0.5 pg bmp2b mRNA at the one-cell stage. (B-D) Embryos were treated with 10 μM FGF inhibitor SU-5402 (B), 50 μM Nodal inhibitor SB-505124 (C), or both (D) starting at 2 hpf. See the Figure 7—figure supplement 3—source data 1 file for source data.

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