ZFIN Image: Zhang et al., 2020, Figure 7

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Figure Caption

Figure 7 Identification of the enhancer activity and analysis of putative TFBSs.

Enhancer activity identification of tnni1b-ECR183 and tnni1b-ECR183-d2 by the luciferase assay in the HEK293 (A) and HL1 cell lines (B). (C) Putative TFBS positions and mutations. Analysis of the enhancer activity of tnni1b-ECR183-d2 after putative TFs were overexpressed (D) or after NKX2.5 (E) and JUN (F) binding sites were mutated. One-way ANOVA was used to test for homogeneity of variance, and Bonferroni’s test was used to correct for multiple comparisons (A, B, D). A t-test was performed for statistical analyses between two groups (E, F). * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001, n = 3.

Acknowledgments

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