Figure 6

Figure 6

Spatiotemporal Acetylcholinesterase enzymatic activity pattern in the spinal cord during early development. (A, B) Lateral view of 24 hpf embryos processed with Karnovsky staining showing Acetylcholinesterase enzymatic activity (bracket) in the absence (A), but not in presence (B) of eserine, an AChE inhibitor. (C) Schematic representation of the imaged areas in (D–F′). Lateral view of 6-day-old larvae showing a rostro-caudal gradient of AChE activity revealed by Karnovsky staining (D–F′). (D, D′) Anterior trunk in the absence (D) or presence (D′) of eserine. (E) Mid trunk in the absence of eserine. (F, F′) Tip of the tail in the absence (F) or presence (F′) of eserine. Brackets indicate high levels of Karnovsky staining in (E, F). Magnification is the same for all images in (A, B) and (D–F′). Scale bar: 200 μm.