ZFIN Image: Satapathy et al., 2020, Fig. 5

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Figure Caption

Fig. 5 GLI1 negatively regulates the differentiation and promotes the proliferation of colon cancer cells in the absence of 15-PGDH.

HT-29 cells were either transfected with shCTRL alone or co-transfected with shHPGD and pEGFP-hGLI1 followed by LTC₄ stimulation for 48 h. qRT-PCR analysis of the differentiation markers aSI, bMUCIN-2 and cCDHR2, d the differentiation regulation marker CDX2, e the Wnt activation marker AXIN2, f the pro-oncogene cMYC and g the proliferation marker CCND1. h Western blot analysis of whole-cell lysates for 15-PGDH, GLI1, and SI expression. α-Tubulin was used as the loading control. i Immunofluorescence analysis of GLI1 and Mucin-2 in unstimulated or LTC₄-stimulated cells transfected with shHPGD or shGLI1. HPRT1 was used as the housekeeping gene for normalization of the qRT-PCR gene expression data. Graphs represent data from 3 to 4 independent experiments and represent the mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001.

Acknowledgments

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