ZFIN Image: Yang et al., 2020, Figure 1-S4

Image

Figure Caption

Figure 1-S4 Macrophages infiltrate the liver through vasculature and vasculature-independent routes.

Double transgenic zebrafish expressing the macrophage mpeg1:GFP and endothelial kdrl:mCherry reporters were used to localize infiltrating macrophages after brain-LPS injection at 8–10 hpi in the four dpf zebrafish. (a–f) Representative images corresponding to Video 3. a, b, c 3D volumetric view of whole liver (demarcated by a dotted line) and surrounding region taken from three timepoints of a confocal time-lapse imaging (see Video 3) of an 80 µm z-stack at 40x. d, e, f High magnification of the boxed region shown in the corresponding left panels. Representative infiltrating macrophages are labeled numerically 1–5 with their relative position to the vasculature: inside (white arrow), associated (blue arrow), or independent of vasculature (yellow arrow).

Acknowledgments

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