ZFIN Image: Esa et al., 2020, Figure 5

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Figure Caption

Figure 5

MetAp2 inhibition blocked tube formation in VECs and LECs. (A) MetAp2 inhibition impaired HUVECs’ and HMVEC-dLyAd cells’ ability to form tubes. HUVECs and HMVEC-dLyAd cells were seeded on Matrigel and incubated for 14 h under different concentration treatments of TNP-470 (0–50 µM). When no TNP-470 was present, extensive neovascularization was clearly visible and an organized capillary network was formed. When the dose of TNP-470 was elevated to 1–50 μm, the cells’ ability to migrate and reorganize into tubes was impaired. n = 8. (B,C) Metap2 inhibition resulting from exposure to TNP-470 caused a reduction in the relative vessel length and an increase in the relative number of end points in both cell lines, as quantified using the AngioTool image analysis software. n = 8. * p < 0.05, *** p < 0.001. The results are presented as mean ± SEM. Scale bar = 200 μm.

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