Figure 4

Figure 4

The effects of H19 and miR-675 on the expressions of the epithelial-to-mesenchymal transition (EMT) markers. (A) EMT protein expressions in the MCF-7 control (mock) or H19-stably overexpressing cells (H19), determined by Western blot analysis. (B) EMT protein expressions in SUM159PT-pH19-mCherry^neg (mCherry^neg) or SUM159PT-pH19-mCherry^high (mCherry^high), determined by Western blot analysis. (C) EMT protein expressions in the MDA-MB-231 control (mock) or H19-stably overexpressing cells (H19), determined by Western blot analysis. (D) EMT protein expressions in the MDA-MB-231 control (mock) or miR-675-stably overexpressing cells (miR-675), determined by Western blot analysis. For each panel, actin was used as the equi-loading control. The relative signal intensities were quantified by ImageJ and shown above the protein bands for the representative experiment figure. The quantification of the triplicate is figured in the graph beside. * p < 0.05; ** p < 0.01; *** p < 0.001; ns: not significant. Uncropped blots are shown in Figures S1–S22.