ZFIN Image: Carrington et al., 2020, Figure 2

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Figure 2

Selection of targets and strategy for assessment of base editing efficiency. (A) Evaluation of sgRNA targets by CRISPR-STAT. Peak profiles from representative wildtype (uninjected) or sgRNA-injected embryos are shown. twist2-T1 and ntl sgRNAs showed multiple peaks indicating DSB/NHEJ repair, whereas twist2-T2 showed no DSB/NHEJ activity. (B) Schematic of workflow for screening zebrafish at the somatic and germline levels. Injected embryos were screened using sequencing and EditR analysis for somatic base editing and additional progeny were grown to adulthood. Adult fish were then screened for germline transmission by outcrossing with wildtype fish and screening by sequencing pools of four embryos followed by confirmation of base editing in individual embryos.

Acknowledgments

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