Figure 7

Figure 7

PCNA immunostaining in the anterior (AI) and mid (MI) intestine of ctrl (control zebrafish), D.I.O. (diet-induced obesity zebrafish), D.I.O. flw 3,5–T2 (D.I.O. zebrafish followed by 3,5–T2), D.I.O. with 3,5–T2 (D.I.O. zebrafish treated with 3,5–T2). (A) AI of ctrl zebrafish (B) AI of D.I.O. (C) AI of D.I.O. flw 3,5–T2. (D) AI of D.I.O. with 3,5–T2. (E) MI of ctrl zebrafish. (F) MI of D.I.O. (G) MI of D.I.O. flw 3,5–T2. and (H) MI of D.I.O. with 3,5–T2. The arrows indicate PCNA positive cells in the folds. (I,J) Bar graphs showing PCNA optical density in the (I) AI and (J) MI of ctrl, D.I.O., D.I.O. flw 3,5–T2 and D.I.O. with 3,5–T2. Data are expressed as mean ± SE. ** p < 0.001, * p < 0.05 compared to the control group. ° p < 0.05 compared to D.I.O. Scale bar: 50 μm in the low magnification and 25 μm in the higher magnification in the boxes.