Figure 5

Figure 5

Calnexin immunostaining in the anterior (AI) and mid (MI) intestine of ctrl (control zebrafish), D.I.O. (diet-induced obesity zebrafish), D.I.O. flw 3,5–T2 (D.I.O. zebrafish followed by 3,5–T2), D.I.O. with 3,5–T2 (D.I.O. zebrafish treated with 3,5–T2). (A) AI of ctrl zebrafish (B) AI of D.I.O. (C) AI of D.I.O. flw 3,5–T2. (D) AI of D.I.O. with 3,5–T2. (E) MI of ctrl zebrafish. (F) MI of D.I.O. (G) MI of D.I.O. flw 3,5–T2. and (H) MI of D.I.O. with 3,5–T2. The arrows indicate calnexin immunoexpression in the enteroendocrine and goblet cells. (I,J) Bar graphs showing calnexin optical density (O.D.) in the (I) AI and (J) MI of ctrl, D.I.O., D.I.O. flw 3,5–T2 and D.I.O. with 3,5–T2. Data are expressed as mean ± SE. ** p < 0.001, * p < 0.05 compared to the control group. ° p < 0.05 compared to D.I.O. Scale bar: 100 μm in the low magnification and 50 μm in the higher magnification in the boxes.