Figure 2

Figure 2

Hematoxylin and eosin (H&E) staining of anterior (AI) and mid (MI) intestine of ctrl (control zebrafish), D.I.O. (diet-induced obesity zebrafish), D.I.O. flw 3,5–T2 (D.I.O. zebrafish followed by 3,5–T2), D.I.O. with 3,5–T2 (D.I.O. zebrafish treated with 3,5–T2). (A) AI and (B) MI of control zebrafish. (C) AI and (D) MI of D.I.O. (E) AI and (F) MI of D.I.O. flw 3,5–T2. (G) AI and (H) MI of D.I.O. with 3,5–T2. Arrows indicate goblet cells, arrowheads indicate ragged villi. (I,J) Bar graphs showing the number of goblet cells in the (I) AI and (J) MI of ctrl, D.I.O., D.I.O. flw 3,5–T2 and D.I.O. with 3,5–T2. Goblet cells were counted based on Alcian Blue staining. Data are expressed as mean ± SE. *** p < 0.0001, * p < 0.05 compared to the control group. ° p < 0.05 compared to D.I.O. Scale bar: 100 μm.