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Figure 6—figure supplement 1.

ID: ZDB-IMAGE-200731-1
Source: Figures for Hsieh et al., 2020

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Figure Caption

Figure 6—figure supplement 1. iR4H-expressing Purkinje cells die during cerebellar development in vivo.

(A) A live image of a Purkinje cell expressing iR4H and membrane-bound tdTomato (mTomato) is shown at 4 dfp. Scale bar = 5 μm. (B) At 5 dpf, the zebrafish was incubated in acridine orange and the cell was re-imaged. Left, mTomato; middle, acridine orange; right, merged image. In apoptotic cells, acridine orange appears as green puncta, which corresponds to the dye binding to condensed, fragmented chromatin (arrows) (Atale et al., 2014). Less intense green staining is present in nearby healthy cells that are not expressing iR4H and do not contain condensed, fragmented chromatin (Atale et al., 2014). Scale bar: 5 μm.

Acknowledgments

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