ZFIN Image: von der Heyde et al., 2020, Figure 5

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Figure 5

The effect of mutations in hcn4 and treatment with ivabradine on (change in) heart rate variability and heart rate. Effects were examined by comparing embryos with nonsense mutations in both hcn4 alleles and embryos free from CRISPR/Cas9 or sa11188 mutations; as well as using an additive model, weighing the number of mutated alleles by their predicted effect on protein function based on Ensembl’s Variant Effect Predictor (VEP). Effects were adjusted for time of day and the weighted number of mutated alleles in other targeted genes as fixed factors, and with embryos nested in batches and experiment (for the combined analysis, random factor). Dots and whiskers represent effect sizes and 95% confidence intervals.

Acknowledgments

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