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Fig. 4 Retinal function of cacna2d4-KO lines at 20 dpf. (A–C) Box and whisker plots of white-light ERG b-wave amplitudes, b-wave kinetics and flicker fusion threshold of 20 dpf WT and double-KO. Bottom and top of the box: the first and third quartile; median, line within the box; whiskers, minimum and maximum values; circles, outliers (1.5 × interquartile distance); small asterisks, extreme outliers (3 × interquartile distance). (A, B) Five flashes (100 ms) of increasing light intensities were applied, starting from dim light (log-4) to 100% light intensity (log0, 24,000 μW/cm2), and responses were grouped accordingly (n = 19 per genotype). (A) b-wave amplitude of the mixed rod-cone response was not attenuated in double-KO. (B) The time from baseline to peak is significantly prolonged in double-KO at almost all light intensities. (C) The flicker fusion threshold is reduced in double-KO under photopic conditions (n = 25 per genotype). Significance levels: *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001.