IMAGE

FIGURE 2

ID
ZDB-IMAGE-200613-2
Source
Figures for Peng et al., 2020
Image
Figure Caption

FIGURE 2

Wnt2bb mediates zebrafish heart regeneration. (A,B) Acid fuchsin orange G (AFOG) staining analyses reveal defective wound healing after ventricular resection in Tg(hsp70l:dn-wnt2bb) fish compared to that observed in the wild-type (ctrl) fish. Red arrow, fibrin; Blue, collagen; HS, heat shock of Tg(hsp70l:dn-wnt2bb) fish at 37°C for 1 h every 2 days for 30 days. (E) Bar charts showing the quantification of AFOG staining from (A,B). (C,D,F) AFOG staining and quantification reveals increased wound healing after ventricular resection in Tg(hsp70l:wnt2bb) fish compared to that observed in the wild-type (ctrl) fish. Red arrow, fibrin; Blue, collagen; HS, heat shock of Tg(hsp70l:wnt2bb) fish at 37°C for 1 h every 2 days for 21 days. Six hearts were assayed per group. (H–J) Confocal microscopy image analyses of PCNA+Mef2C+ cells (arrowheads) in heat-shocked Tg(hsp70l:dn-wnt2bb), Tg(hsp70l:wnt2bb) and wild-type (ctrl) fish at 7 dpa. Green, PCNA; Red, Mef2C. Boxes correspond to the magnified region. (K) Bar charts showing the quantification of PCNA-labeled CM proliferation index values for heat-shocked, injured wild-type (ctrl) and Tg(hsp70l:dn-wnt2bb) and Tg(hsp70l:wnt2bb) fish hearts. (G) Schematic of the experimental plan to analyze PCNA/Mef2c proliferation after apex amputation. The data are presented as the means ± SEM from 5 to 7 hearts for each group. The proliferation data were collected for 4–6 sections per heart and averaged to generate each data point. Error bars: ± 1 SD. Significance was determined using Student’s t-test: **P < 0.01, ****P < 0.0001. Brackets indicate the amputation area. Ctrl, control. Scale bar, 100μm.

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Cell Dev Biol