IMAGE

FIGURE 1

ID
ZDB-IMAGE-200613-1
Source
Figures for Peng et al., 2020
Image
Figure Caption

FIGURE 1

Induction of jnk1, creb1, wnt2bb and c-jun expression in injured cardiomyocytes during zebrafish heart regeneration (A) RT-qPCR results showing changes in wnt2bb creb1 nkx2.5 and c-jun expression at 4 and 7 dpa. (B,C) Confocal microscopy images showing creb1 expression during heart regeneration. At 7 dpa, creb1 expression expanded to cardiomyocytes adjacent to the injury site. Green, creb1; Red, MHC. (E,F) Confocal microscopy images of jnk expression after cardiac injury. The expression of jnk was low in uninjured ventricles, but at 7 dpa, jnk expression was enhanced and expanded into the new cardiomyocytes at the wound site (arrows). Green, jnk; Red, MHC. (H) Whereas wnt2bb expression was detectable on the uninjured ventricle (I), wnt2bb expression was enhanced in CMs adjacent to the injury site, and some non-CMs expressed wnt2bb at the apical edge of the wound at 7 dpa. Green, wnt2bb; Red, MHC. (K,L) Immunostaining analyses showed increased c-jun expression at the apical cell edges of the wounded heart and gradually increased at 7 dpa. Green, c-jun; Red, MHC. (D,G,J,M). Bar graph showing the fold changes in creb1, c-jun, jnk1, and wnt2bb expression in cardiomyocyte at 7 dpa compared to that observed in the uninjured heart. The data are presented as the means ± SEM, and significance was determined using Student’s t-test. n = 5. Brackets indicate the amputation area. Boxes correspond to the magnified region in the adjacent panels [Scale bars: 100 μm (left) and 25 μm (right)].

Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Cell Dev Biol