Fig. 6

Fig. 6

a Representative images and b quantification of subintestinal veins (SIVs) development alteration, evidenced by whole-mount alkaline phosphatase staining in Casper zebrafish embryos at 72 hpf exposed to 1 and 2 μM CPTH6 at 6 hpf. c Representative images and (d) quantification of SIVs development alteration evidenced by whole-mount alkaline phosphatase staining in Casper zebrafish embryos at 72 hpf injected in the yolk with 50 μM CPTH6 solution at 48 hpf. b,d Development alteration was evaluated in terms of sprouting embryos (100%), sproutings/embryo, intersections/embryo. The error bar represents the standard error of the mean (SEM) of 3 independent experiments (N = 75 larvae/experiment). e Representative images of GFP-positive ISVs development and Dsred-positive erythroid cells and f percentage of larvae with altered circulation in Tg (kdrl: GFP;gata1:dsRed) Casper zebrafish at 72 hpf exposed to 1 and 2 μM CPTH6 at 6 hpf. At higher magnification the accumulation of gata1-positive erythroid cells is evidenced in caudal erythropoietic tissue of CPTH6-treated embryos. f A representative experiment of 4 independent experiments (n = 30–40 larvae/experiment) with the same result is shown. g Bright-field micrographs of Casper zebrafish larvae at 96 hpf exposed to 1 and 2 μM CPTH6 at 6 hpf. Arrow indicates the normal color of blood. Scale bar, 200 μm. a-g As control, zebrafish larvae were exposed to DMSO solution. b,d,f *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. (a, c, e) Scale bar, 100 μm