ZFIN Image: Keomanee-Dizon et al., 2020, FIG. 1.

Image

Figure Caption

FIG. 1.

Light-sheet microscopy principle. A light-sheet (blue) can be created by dynamically scanning, along the y direction, a focused Gaussian beam that propagates in the x direction. The focusing is achieved via a low numerical aperture illumination lens. The fluorescence generated by the illuminated (x–y) plane is imaged (green) by an orthogonally positioned wide-field microscope. Axial (x–y) sections of the 3D sample are captured either by scanning the sample (orange) through the stationary focal plane or by scanning the light sheet and detection focal plane through the stationary sample.

Acknowledgments

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