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Fig. 8 Validation of hs:dkk1b RNA-Seq analysis. Embryos collected from a hs:dkk1b/+ X +/+ cross were heat shocked during gastrulation according to experimental regimens outlined in Fig. S5 and fixed at 7 hpf (5→7 hpf experiment) or 9 hpf (7→9 hpf experiment) for in situ hybridization. Gene analyzed is indicated on left. Fold change is from the RNA-seq analysis (Supplemental Tables 1 and 2). wt: +/+ sibling. Tg: hs:dkk1b/+ embryo. wt/Tg: representative embryo from collection, genotype cannot be determined by staining pattern. Animal pole up, lateral views (A–D) or dorsal views (E–J).
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Reprinted from Developmental Biology, 462(2), Green, D.G., Whitener, A.E., Mohanty, S., Mistretta, B., Gunaratne, P., Yeh, A.T., Lekven, A.C., Wnt signaling regulates neural plate patterning in distinct temporal phases with dynamic transcriptional outputs, 152-164, Copyright (2020) with permission from Elsevier. Full text @ Dev. Biol.