ZFIN Image: Green et al., 2020, Fig. s5

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Figure Caption

Fig. s5 Schematic outline of embryo production procedure for gastrula stage Wnt modulation RNA-Seq analysis. hs:dkk1b/+ and hs:wnt8a/+ transgenic fish were crossed to WT strains. Embryos were collected and heat shocked two time points, 5/5.5 hpf (time 1) or 7/7.5 hpf (time 2) for hs:dkk1 and hs:wnt8a respectively. Embryos were sorted into GFP+ (transgenic embryos) and GFP- (wild-type sibling embryos) pools and homogenized in Trizol at 7 hpf (time 1) or 9 hpf (time 2). Total RNA was isolated and submitted to the Texas A&M AgriLife Research Genomics and Bioinformatics Service for library preparation and Illumina sequencing (See Material and Methods for more details).

Acknowledgments

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Reprinted from Developmental Biology, 462(2), Green, D.G., Whitener, A.E., Mohanty, S., Mistretta, B., Gunaratne, P., Yeh, A.T., Lekven, A.C., Wnt signaling regulates neural plate patterning in distinct temporal phases with dynamic transcriptional outputs, 152-164, Copyright (2020) with permission from Elsevier. Full text @ Dev. Biol.