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Fig. s3 Wnt suppression during morphogenesis phase does not inhibit the MHB GRN before 19 hpf. In situ hybridizations for fgf8a, pax2a, and wnt1 on embryos from hs:dkk1b/+ X +/+ crosses, heat shocked at 14 hpf, and analyzed at the indicated times. No clear differences in gene expression between hs:dkk1b/+ and +/+ embryos could be observed at either 17 or 19 hpf.
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Reprinted from Developmental Biology, 462(2), Green, D.G., Whitener, A.E., Mohanty, S., Mistretta, B., Gunaratne, P., Yeh, A.T., Lekven, A.C., Wnt signaling regulates neural plate patterning in distinct temporal phases with dynamic transcriptional outputs, 152-164, Copyright (2020) with permission from Elsevier. Full text @ Dev. Biol.