ZFIN Image: Green et al., 2020, Fig. 2

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Figure Caption

Fig. 2 Wnt suppression transiently disrupts MHB establishment during epiboly. In situ hybridization to fgf8a (A–D), pax2a (E–H), and otx2b (I–L). hs:dkk1b/+ or +/+ sibling (control) embryos heat shocked at 4.7 hpf, then analyzed at 9 hpf (A,B,E,F,I,J) or 10.5hpf (C,D,G,H,K,L). (A–H) Dorsal view, animal pole up. (I–L) Lateral view, animal pole up. Note the absence of MHB associated fgf8a and pax2a at 9 hpf (B,F, arrows) but return of expression by 10.5 hpf (D,H). Arrows in (I–L) indicate anterior limit of otx2b, posterior limit of otx2b, and posterior edge of embryonic margin. Note shortened distance between the otx2b posterior limit relative to the margin in conditions of Wnt loss of function.

Acknowledgments

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Reprinted from Developmental Biology, 462(2), Green, D.G., Whitener, A.E., Mohanty, S., Mistretta, B., Gunaratne, P., Yeh, A.T., Lekven, A.C., Wnt signaling regulates neural plate patterning in distinct temporal phases with dynamic transcriptional outputs, 152-164, Copyright (2020) with permission from Elsevier. Full text @ Dev. Biol.