ZFIN Image: Kara et al., 2019, Fig. S2

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Figure Caption

Fig. S2

miR-216a mimic and Dot1l morpholino injections do not lead to increased apoptosis in the retina. Related to Figure 1 and Figure 4. (A) Control miRNA or miR-216a or (C) control morpholinos or Dot1l morpholinos were injected and electroporated into the left eyes of Tg(gfap:GFP) zebrafish before intense light exposure (0h). After 45h, retinas were collected, sectioned and TUNEL labeling was performed along with anti-GFP immunostaining. Nuclei were counterstained with TOPRO (blue). (B, D) TUNEL-positive cells in the INL were quantified. Data represent mean +/- SEM, n=7-9 fish. Two-tailed, Mann–Whitney U test is performed. ONL, Outer nuclear layer; INL, inner nuclear layer; GCL, ganglion cell layer. Scale bar 50um.

Acknowledgments

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