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Fig. 6
miR-216a Gain of Function Impairs β-Catenin Accumulation in MG after Intense Light Damage
(A) Control miRNA or miR-216a was injected and electroporated into the left eyes of wild-type zebrafish before intense light exposure (0 h). After 45 h, retinas were collected, sectioned, and immunostained using antibodies against β-catenin (red) and PCNA (green). Nuclei were counterstained with TOPRO (blue).
(B) β-Catenin colocalized with PCNA+ proliferating MG after 45 h of intense light damage. β-Catenin accumulation was not detected in miR-216a-overexpressing retinas. Scale bar, 50 μm.
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