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Fig. S3
Images of engulfed apoptotic neurons at 3 dpf
Compound transgenic reporter strain zebrafish embryos Tg:(PU1:GFP)/Tg:(NBT:dsRed) were analyzed by in vivo CLSM at 3 dpf. Representative projections of confocal z-stacks through the brain: A, D: NBT:dsRed (neurons); B ,E: PU1:GFP (microglia). C, F: overlays of the respective panels (A,B and C,D). Boxed regions, increased images: a., d.: apoptotic neurons; b., e.: microglia; c. f.: microglia with engulfed apoptotic bodies of neurons (= loaded microglia; arrows). Size markers 40 and 10 μm.
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