ZFIN Image: Shi et al., 2020, Fig. 7

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Figure Caption

Fig. 7 Visualization of combinatorial expression on Zebrabow samples.

Maximum intensity projection renderings of Tg(ubi:Zebrabow) muscle³⁴ acquired live in multispectral confocal mode with 458 nm excitation. a The elicited signal (e.g., white arrows) is difficult to interpret in the TrueColor Image display (Zen Software, Zeiss, Germany). b Discerning spectral differences is increasingly simpler with gradient descent map scaled to intensities, while compromising on the brightness of the image. c Gradient descent and d gradient ascent RGB masks in scale mode show the color values assigned to each pixel and greatly improve the visual separation of recombined CFP, YFP, and RFP labels. Colorbars represent the main wavelength associated to one color in nanometers.

Acknowledgments

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