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Figure 5-figure supplement 3 Intracellular calcium measurement in C2C12 myotubes.

Ratiometric fura-2 imaging with 10 mM caffeine induction after treatment with A) SB203580, (B) SB202190, and C) p38 MAPK siRNAs. Black lines indicate individual myofibers while red lines present the mean ± SEM as show in Figure 5. Ratiometric 340/380 data were normalized to the average of 10 s of baseline resting free Ca2+ concentration ([Ca2+]i), that is normalized Δ ratio.

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