ZFIN Image: Volpatti et al., 2020, Figure 5-figure supplement 2

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Figure Caption

Figure 5-figure supplement 2 Quantitative PCR analysis was performed to validate the efficiency of siRNA knockdown of three p38 MAPK genes in both WT and Ryr1 KO myotubes.

As shown, the siRNAs reduce target gene expression by >50% in WT cells (A) while the degree of knockdown achieved in Ryr1 KO myotubes (B) was more variable. Data are presented as mean ± SEM fold change for n = 3 independent myotube cultures. Statistical analysis by one-way ANOVA followed by Holm-Sidak’s multiple comparisons post-test to compare each group to the negative control siRNA. *p<0.05, **p<0.01, ***p<0.001.

Acknowledgments

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