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Figure 2-figure supplement 4 Follow-up testing for compounds identified in the primary screen.

(A) Chemical dose-response re-testing of five compounds shows consistent ability to suppress nemadipine-A-induced growth arrest of unc-68 mutants. Note: wact-526 precipitated at 30 and 60 μM and rand-62 precipitated at 60 μM. (B–G) Post-hoc statistical analysis showing the developmental stage distributions of the 145 re-tested molecules from the primary screen, and identifying those that were significantly different from DMSO wells (red arrows). Again, it is important to note that this was performed while assuming that 20 worms were in each well as a means of estimating the proportion of actual L4 and adult counts in each well. In other words, we assumed #L1-L3 larvae = 20 – (#L4 + #adult). Plots shown are from independent screens of (B) 7.5 μM wactives, (C) 60 μM wactives, (D) 60 μM ‘random’ non-wactives, (E) 60 μM US Drug Collection and (F-G) 60 μM GlaxoSmithKline Published Kinase Inhibitor (PKI) set. In the PKI set, p38 inhibitors that suppressed growth arrest are highlighted by green arrows. Statistical analysis by Kruskal-Wallis test with Dunn’s multiple comparisons post-test where *p<0.05, **p<0.01, ***p<0.001.

Acknowledgments
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