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FIGURE 1

Germline specific dgcr8 deletion has no functional influence on gonad development. (A) A basic schematic diagram of generating dgcr8 germline conditional knockout zebrafish. (B) Real-time analysis of the expression of dgcr8 mRNA and primary miRNAs in ovary of dgcr8 kop-cKO fish. (C) Real-time PCR analysis of the expression of mature miRNAs in ovary of dgcr8 kop-cKO fish. (D) Expression of germline markers in the ovary of dgcr8 kop-cKO. (E) Histology of ovaries in the wild-type and dgcr8 kop-cKO female zebrafish. *p < 0.05, **p < 0.01, and ****p < 0.0001.

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