ZFIN Image: Sensi et al., 2020, Figure 1

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Figure 1

Characterization of matched 3DN and 3DT HT29-recellularized samples. (A) histological characterization of sections stained with hematoxylin and eosin (H&E), collagen IV staining (Col IV), and periodic acid-Shiff (PAS) (scale bar = 200 µm). Scanning electron microscopy (SEM) analysis performed on 3DN and 3DT (Scale bar = 10 µm). (B) DNA amount quantification in fresh samples, after decellularization and 5 days of culture after seeding of HT29 cells, in both 3DN and 3DT. (C) IF staining in 3DT and 3DN and relative quantifications: Ki67, as proliferation marker; E-cadherin, as epithelial marker; vimentin, as mesenchymal marker; laminin to highlight basement membrane; DAPI to counterstain the nuclei (scale bar = 100 µm) (* p-value < 0.05; ** p-value < 0.01).

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