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Figure 7
FH decreased tumor angiogenesis in TNBC xenograft zebrafish. 200 TGFβ1 48 h treated TNBC cells labeled with a red fluorescent dye (CM‐DiI) were injected into 2dpf normal transgenic vascular green fluorescent zebrafish yolk sac. After xenograft tumor model was established, the tumor models were treated with FH‐L, FH‐M, FH‐H, CDDP, and control. The fluorescence images were analyzed by a laser confocal scanning microscope. The representative pictures of subintestinal vessel sprout were observed with the laser confocal scanning microscope (original magnification, 200×). White solid arrow indicates sprouts of subintestinal blood vessels. The red is the MDA‐MB‐231 cells of TGFβ1‐treated and labeled with CM‐DiI. The number of buds in the intestine was used to evaluate the inhibition of FH on neovascularization. Normal represents normal transgenic vascular green fluorescent zebrafish, Mod represents the TNBC xenograft tumor model in zebrafish, thalidomide represents thalidomide (219 μg/mL), FH‐L represents Fangjihuangqi Decoction (55.5 μg/mL), FH‐M represents Fangjihuangqi Decoction (111 μg/mL), and FH‐H represents Fangjihuangqi Decoction (222 μg/mL). **