Figure 5

Figure 5

Smoc1 Mutants Scaling Phenotype

(A) smoc1 genomic structure. Exon colors match the protein domains they encode: SP, signal peptide; FS, follistatin-like domain; Thy, thyroglobulin-like domain; EC, extracellular domain. Target sequence of the Smoc1^ug104 CRISPR mutant is located in exon 4; mutant sequence of Smoc1^ug104, highlighted in magenta. Smoc1^ug104 results in a truncated protein, which lacks the first Thyroglobulin domain and other downstream features. The position of the splicing morpholino and Smoc1 antibody epitope region are indicated.

(B and C) BRE:GFP gradients in Smoc1 homozygote mutants (C) and heterozygote controls (B) displayed as straightened ROI, as in Figure 1D (anterior, left).

(D and E) Decay length versus ROI half-length (L) of BRE:GFP gradients in Smoc1^−/− mutants (black) and wild-type controls (green, from Figures 3C and 3D). Dots, average from length bins ± SEM (for individual data points, see Figures S4W–S4X). Lines, linear fits to individual data. Slope (φ) values ± SEM are shown.

(F) Comparison of φ=λ/L from BRE:GFP gradients of wild-type, Smoc1^−/− mutants and Smoc1^−/− mutants injected with smoc1 mRNA (“rescue”).

(G–I) Scanning electron micrographs of wild-type (G), Smoc1 heterozygote control (H), and Smoc1 homozygote fins (I).

(J and K) Average PD (J) and AP (K) fin lengths at 78 hpf in different mutant and morphant (MO) conditions.

(L) Comparison of average goodness of fit, R2(R‾2) obtained from collapse analysis (see Figures S4A–S4H) of BRE:GFP gradients in wild-type or Smoc1^−/− mutants. n represents number of fins analyzed.

For all statistical analyses: ^∗∗∗p < 0.0001; ^∗∗p < 0.01, ^∗p < 0.05; two-tailed, unpaired, non-parametric Mann-Whitney tests. Mean ± SEM are shown in all graphs. Anterior, left; distal down. Scale bars, 50 μm. BRE:GFP transgene used: BRE:eGFP (Laux et al., 2011) (B–F, L). See also Figures S4 and S5.