ZFIN Image: Lee et al., 2020, Figure 5

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Figure 5

Anti-osteoporotic effect of LEA in a model of prednisolone-induced osteoporosis in zebrafish. (A) The larvae at 10 dpf (days post-fertilization) were treated with 25 µM prednisolone (PS) in the presence or absence of LEA (10 μg/mL) for 3 days. Whole-mount Alizarin red staining was performed to analyze the mineralized bone. Arrowheads indicate the bone Alizarin red staining. (B) Relative vertebral bone density was assessed by measuring the areas of the first ten stained vertebrae (V1–V10, indicated by arrowhead in (A)). *** p < 0.001 versus only prednisolone treatment.

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