ZFIN Image: Lee et al., 2020, Figure 4

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Figure Caption

Figure 4

LEA suppresses NFATc1 expression by blocking both NF-κB and NFATc1-mediated transactivity. (A) BMMs were pre-treated with DMSO and LEA (10 μg/mL) for 1 h, and stimulated with RANKL for 0, 5, 15, and 30 min. The cells were lysed and immunoblotted with antibodies against p-Akt, Akt, p-ERK, ERK, p-IκB, IκB, p-p38, p38, p-JNK, and JNK. Band intensities were normalized to β-Actin control. (B) 293T cells were transiently transfected with the reporter plasmid Nfatc1-Luc along with c-Fos, p65, or Nfatc1 in the presence or absence of LEA (10 μg/mL). Luciferase activity was measured after 24 h post-transfection using Luciferase assay kit (Promega, Madison, WI, USA). Each bar represents the means ± S.D. of three independent experiments. *** p < 0.001. ns = not significant.

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